P84單克隆抗體，也被稱為CD172a抗體，可與信號調節蛋白α(SIRPα)反應。SIRPα蛋白是I型跨膜糖蛋白，在單核細胞、巨噬細胞和樹突狀細胞均有表達。此外，在神經元和中樞神經系統的一些其他組織也發現有SIRPα蛋白的表達。其配體CD47在多種細胞均有表達。
SIRPα和CD47參與調節由樹突狀細胞介導的T細胞的活化、中性粒細胞遷移和吞噬等過程。SIRPα蛋白可在巨噬細胞的細胞膜上進行橫向擴散并在吞噬性突觸中積累，與CD47結合后，抑制巨噬細胞的吞噬作用。
研究結果表明，使用anti-SIRPα抗體阻斷SIRPα與CD47相互作用，可抑制小鼠體內腫瘤的形成。此外，P84（CD172a）單克隆抗體在體內和體外均具有中和活性。
BioXcell最新推出InVivoMAb anti-mouse CD172a (SIRPα)單克隆抗體，助力腫瘤免疫檢查點研究。如需購買BioXcell公司產品，請聯系代理商欣博盛生物。
產品信息：
產品貨號 | BE0322 |
產品名稱 | InVivoMAb anti-mouse CD172a (SIRPα) |
規格 | 1mg,5mg,25mg, 50mg, 100mg |
克隆號 | P84 |
同種型(Isotype) | Rat IgG1, κ |
免疫原(Immunogen) | Mouse brain membrane protein |
成分(Formulation) | PBS, pH 7.0 |
Contains no stabilizers or preservatives | |
內毒素(Endotoxin) | <2EU/mg (<0.002EU/μg) |
Determined by LAL gel clotting assay | |
純度(Purity) | >95% |
Determined by SDS-PAGE | |
無菌(Sterility) | 0.2 μM filtered |
生產(Production) | Purified from tissue culture supernatant in an animal free facility |
純化(Purification) | Protein A |
保存(Storage) | Undiluted at 4°C in the dark |
應用(Reported Applications) | In vivo SIRPα blocking |
In vitro SIRPα blocking | |
Western blot |
Application References:
Yanagita, T., et al. (2017). 'Anti-SIRPalpha antibodies as a potential new tool for cancer immunotherapy.' JCI Insight 2(1): e89140.
Koskinen, C., et al. (2013). 'Lack of CD47 impairs bone cell differentiation and results in an osteopenic phenotype in vivo due to impaired signal regulatory protein alpha (SIRPalpha) signaling.' J Biol Chem 288(41): 29333-29344.
Teraoka, Y., et al. (2013). 'Expression of recipient CD47 on rat insulinoma cell xenografts prevents macrophage-mediated rejection through SIRPalpha inhibitory signaling in mice.' PLoS One 8(3): e58359.
Zen, K., et al. (2013). 'Inflammation-induced proteolytic processing of the SIRPalpha cytoplasmic ITIM in neutrophils propagates a proinflammatory state.' Nat Commun 4: 2436.
Lundberg, P., et al. (2007). 'Osteoclast formation is strongly reduced both in vivo and in vitro in the absence of CD47/SIRPalpha-interaction.' Biochem Biophys Res Commun 352(2): 444-448.
Oldenborg, P. A., et al. (2001). 'CD47-signal regulatory protein alpha (SIRPalpha) regulates Fcgamma and complement receptor-mediated phagocytosis.' J Exp Med 193(7): 855-862.
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